HTB-38 HT-29 人結腸癌細胞

ATCC® Number:  HTB-38™

Designations:  HT-29

Depositors:

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:  Isolation date: 1964

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: human adrenergic alpha2A [23560]

urokinase receptor (u-PAR)

vitamin D (moderate expression)

urokinase receptor (u-PAR); vitamin D (moderate expression)

Virus Susceptibility: human immunodeficiency virus (HIV, LAV)

Tumorigenic: Yes

Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -

Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 11,12

D16S539: 11,12

D5S818: 11,12

D7S820: 10

THO1: 6,9

TPOX: 8,9

vWA: 17,19

Cytogenetic Analysis: modal number = 71; range = 68 to 72.

The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.

Isoenzymes:  AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1-2

Age:  44 years adult

Gender:  female

Ethnicity:  Caucasian

Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.

There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

derivative:ATCC CCL-218

purified DNA:ATCC HTB-38D

HTB-38 HT-29 人結腸癌細胞

MLE-12 小鼠肺上皮細胞  

saos-2 人成骨肉瘤細胞

MA104 非洲綠猴(hou)胚胎腎(shen)上皮細胞

ball-1 細胞

nci-h226 人肺鱗癌細胞

MCA38 小(xiao)鼠(shu)結腸癌細胞(bao)

LTPA 小鼠胰腺癌細胞(bao)

NCI-H23 人非小細(xi)胞肺癌細(xi)胞

293.00     人胚腎細胞

293FT    人胚腎細(xi)胞-用于慢病毒包裝

293TN    人胚腎(shen)細胞--用于(yu)慢病毒包(bao)裝(zhuang)

293AV    人(ren)胚腎(shen)細胞-用(yong)于腺病毒包裝

293T    人(ren)胚腎(shen)細(xi)胞

208F    大鼠成纖維細胞

786-O    人腎透明細胞腺癌細胞

A172    人腦膠(jiao)質母細胞瘤細胞

A549    人肺(fei)癌(ai)細(xi)胞 細(xi)胞系購買

AGS    人(ren)胃(wei)癌細胞(bao)

BEL7402 人(ren)肝癌(ai)細胞系

AZ-521    人胃癌細胞

B16F0    小鼠黑(hei)色素(su)瘤(liu)細(xi)胞

Bel-7402    人肝癌(ai)細胞

Bel-7404    人(ren)肝癌細(xi)胞

BGC-823    人胃癌(ai)細(xi)胞

C6    大鼠腦膠質瘤細胞

SW480    人結腸(chang)腺癌細胞(bao)

CaCo-2    人結腸癌(ai)細胞

Cho-K1    倉鼠卵(luan)巢細胞亞株(zhu)

COS-7    非洲綠猴SV40轉化的腎細胞

GES-1    人胃上皮細胞(bao)

HCT-8    人回盲(mang)腸癌細(xi)胞

HeLa

Hepa1-6    小鼠(shu)肝癌細胞

HepG2    人肝癌細胞

hFOB1.19    人永生化成骨細胞(bao)

HGC-27    人胃癌細胞

Hs 746T    人胃(wei)癌細胞

HT-29    人結腸(chang)癌細胞

KB    人口腔表皮樣(yang)癌細(xi)胞(bao)

LoVo    人(ren)結腸癌細胞

LLC-PK1    豬腎近曲小管(guan)上皮細胞

MCF-10A    人正(zheng)常乳腺上皮細胞

MCF-7    人(ren)乳(ru)腺癌細(xi)胞

MDA-MB-231    人乳腺癌細胞

MGC80-3    人胃癌細胞

MKN-45    人胃癌細胞(bao)

NCI-H460    人大細胞(bao)肺癌細胞(bao)

NIH-3T3    小鼠(shu)成纖維細胞

NUGC-3    人胃癌細(xi)胞

Raw264-7    小鼠(shu)單核細胞(bao)(bao)白(bai)血病細胞(bao)(bao)

SGC-7901    人(ren)胃癌細胞

SMMC-7721    人肝癌細胞(bao)

SNU-638    人(ren)胃癌細胞

SNU-739    人肝癌細胞(bao)

Tca8113    人舌(she)鱗癌細胞(bao)    細胞(bao)系購買(mai)

U251    人腦膠質瘤細胞

U-373MG    人腦(nao)神經膠(jiao)質瘤細胞

U-87 MG    人腦(nao)星形膠質母(mu)細(xi)胞瘤

BT-474    人乳腺導管癌細胞

PANC-1    人胰腺癌細胞

HEK293    人胚腎細胞

Hep3B    人肝(gan)癌(ai)細胞

HL-7702    人正(zheng)常(chang)肝細胞

Raw-Blue    小(xiao)鼠單(dan)核細胞(bao)白血病細胞(bao)

WEHI-231    小鼠(shu)B淋巴細(xi)胞

OPM-2    人骨髓瘤細胞

NCI-H929    人骨髓瘤(liu)細胞(bao)

U266    人骨髓瘤細胞

EJ    人膀胱癌(ai)細胞

HOC    大鼠肝卵圓細胞

RWPE-1    人正常前列腺(xian)上皮細(xi)胞

CAL 27    人舌鱗癌細胞(bao)

Calu-3    人肺(fei)腺癌(ai)細胞

Eca-109    人(ren)食管癌細胞

DLD-1    人(ren)結直腸腺癌上皮細胞

5637.00     人膀胱(guang)癌細(xi)胞

SK-OV-3    人卵巢癌(ai)細胞

GBC-SD    人膽囊癌細胞(bao)

BEL7402 人肝癌細胞(bao)系

SW620    人結腸癌(ai)細胞

MRC-5    人胚肺成纖維細(xi)胞     細(xi)胞系(xi)購買

PC9    人肺(fei)癌細(xi)胞

PC14    人(ren)肺(fei)癌細胞

BxPC    人原(yuan)位胰(yi)腺腺癌細胞

PK（15）    豬腎(shen)上(shang)皮(pi)細(xi)胞

MDCK    狗腎上皮細(xi)胞

3D4/21    豬(zhu)肺泡巨(ju)噬(shi)細胞

MHCC-97L    人肝(gan)癌細胞（低轉移）

MHCC-97H    人(ren)肝癌細胞系（高轉移(yi)）

MiaCaPa-2    人胰腺(xian)癌(ai)細胞

HN13    口腔鱗(lin)狀細胞癌細胞

Bend3    小鼠(shu)腦微血(xue)管內皮細胞(bao)

QBC939    人膽管癌(ai)細胞

Ishikawa    人子宮內(nei)膜癌細胞(bao)

A498    人腎(shen)癌細胞

143B

WEHI-231    小鼠B淋巴細胞

BN

P9R    小鼠骨髓基質細胞

P9    小鼠(shu)骨髓基質(zhi)細胞

F98    大鼠腦膠(jiao)質瘤細胞

NCIH1299    人非小細胞(bao)肺癌細胞(bao)

NCIH1650-M3    人非小細胞肺(fei)癌細胞

293LVT    人胚腎細胞--用(yong)于慢病毒包裝

EC9706    人食(shi)管癌細胞

CNE    人鼻咽(yan)癌細胞

H322    人非小細胞(bao)肺癌(ai)細胞(bao)

Panc O2

S8    BEL7402 人肝癌細胞(bao)系

R5    細胞系購買(mai)

Pig MSC    豬骨髓間質干細胞

HCAEC    人冠狀(zhuang)動脈內皮(pi)細胞

marc-145    猴胚(pei)胎(tai)腎上皮(pi)細胞(bao)

3D4/21    豬肺泡巨噬細胞

PK（15）    豬腎(shen)上皮細胞

MADB-106    大鼠乳(ru)腺癌(ai)細胞

Sf9    昆(kun)蟲卵巢細胞

DADY

HUVEC[HUV-EC-C]    人臍靜(jing)脈內皮細胞

PC14    人(ren)肺癌細胞

PC9    人肺癌細(xi)胞

Eca-109    人食(shi)管(guan)癌細胞

CAL 27    人舌鱗癌細胞(bao)

HOC    肝卵圓細胞

EJ    人膀胱癌細胞

U266    人骨髓(sui)瘤細胞

293-mTLR5    人胚(pei)腎(shen)細(xi)胞

Raw-Blue    小(xiao)鼠(shu)單核細胞白血病細胞

HL-7702    人正(zheng)常肝(gan)細胞

Hep3B    人(ren)肝癌細(xi)胞

U-373MG    人腦神經膠質瘤細胞

Tca-8113    人舌鱗癌細胞(bao)

SNU-739    人肝癌細(xi)胞(bao)

SNU-638    人胃癌細胞

NUGC-3    人胃癌細胞

NIH-3T3    小鼠(shu)成纖維細(xi)胞(bao)

MKN-45    人胃(wei)癌細胞(bao)

LLC-PK1    豬腎(shen)近(jin)曲小管上皮(pi)細胞

Hs 746T    人(ren)胃癌細胞

Hepa1-6    小鼠肝癌細胞(bao)

HCT-8    人回盲腸(chang)癌細胞

BEL7402 人肝癌細胞(bao)系

HaCaT    人永生化(hua)表皮細胞

GES-1    人胃上皮細胞

B16-F0    小鼠黑色素瘤細(xi)胞

AZ-521    人胃(wei)癌細胞

786-O    人腎透明(ming)細(xi)胞腺癌細(xi)胞

208F    大(da)鼠成纖維細胞

293TN    人胚(pei)腎細(xi)胞--用于慢病(bing)毒(du)包裝

293FT    人胚腎細胞-用于慢病毒(du)包裝(zhuang)

GP-293    人胚(pei)腎細胞

293-L.P.    人胚腎細胞