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Calu-3 人類肺腺癌細胞（胸水）

簡要描(miao)述：Calu-3 人類肺腺癌細胞（胸水）
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  • 產品(pin)型(xing)號：HTB-55
  • 廠(chang)商性質：生產廠家
  • 更新(xin)時間：2024-03-12
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Calu-3 人類肺腺癌細胞（胸水）

ATCC® Number:HTB-55™    

Designations:Calu-3

Depositors:J Fogh

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Calu-3 人類肺腺癌細胞（胸水）

Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:Blood Type A; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 12,14

D5S818: 11

D7S820: 10,11

THO1: 6,9.3

TPOX: 8

vWA: 16,17



Cytogenetic Analysis:The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1-2



Age:25 years

Gender:male

Ethnicity:Caucasian

Comments:The patient had received prior therapy with cytoxan, bleomycin and adriamycin.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing:Protocol:

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week



MLE-12 小鼠肺上皮細胞

saos-2 人成骨肉瘤細(xi)胞

MA104 非洲綠猴胚胎(tai)腎上(shang)皮細胞

ball-1 細胞

nci-h226 人肺鱗癌細(xi)胞

MCA38 小鼠結(jie)腸癌細胞

LTPA 小(xiao)鼠胰腺癌細(xi)胞(bao)

NCI-H23 人非小細(xi)胞(bao)(bao)肺癌細(xi)胞(bao)(bao)

293.00     人胚腎細(xi)胞

293FT    人胚腎細胞-用于(yu)慢病毒包(bao)裝

293TN    人胚腎細胞--用(yong)于慢病毒包裝

293AV    人胚腎細(xi)胞-用于(yu)腺病毒包裝

293T    人(ren)胚腎細(xi)胞

208F    大(da)鼠成纖維細(xi)胞

786-O    人腎透明(ming)細(xi)胞(bao)腺(xian)癌細(xi)胞(bao)

A172    人腦膠(jiao)質(zhi)母細胞瘤(liu)細胞

A549    人肺癌細(xi)胞(bao) 細(xi)胞(bao)系(xi)購買

AGS    人胃癌(ai)細胞

BEL7402 人肝(gan)癌細胞系

AZ-521    人胃癌(ai)細胞

B16F0    小鼠黑色(se)素瘤細胞

Bel-7402    人肝癌(ai)細胞

Bel-7404    人肝癌(ai)細胞

BGC-823    人胃癌細胞

C6    大鼠腦膠質瘤細胞

SW480    人(ren)結腸(chang)腺癌(ai)細(xi)胞

CaCo-2    人(ren)結腸癌細胞

Cho-K1    倉鼠卵巢(chao)細胞亞株

COS-7    非洲綠(lv)猴SV40轉化的腎細胞

GES-1    人(ren)胃(wei)上皮細胞

HCT-8    人回盲腸癌(ai)細胞

HeLa

Hepa1-6    小(xiao)鼠肝癌細胞

HepG2    人肝癌細胞

hFOB1.19    人(ren)永生化成骨細(xi)胞

HGC-27    人胃癌(ai)細(xi)胞(bao)

Hs 746T    人胃癌細胞(bao)

HT-29    人結腸癌細胞(bao)

KB    人口(kou)腔表(biao)皮樣(yang)癌細胞

LoVo    人(ren)結腸癌細胞

LLC-PK1    豬腎近曲小管上皮細胞

MCF-10A    人正常乳腺上皮(pi)細胞(bao)

MCF-7    人乳腺癌細胞

MDA-MB-231    人乳腺(xian)癌(ai)細胞(bao)

MGC80-3    人胃癌細胞

MKN-45    人胃癌(ai)細(xi)胞

NCI-H460    人大(da)細胞肺癌(ai)細胞

NIH-3T3    小(xiao)鼠成纖維細胞(bao)

NUGC-3    人胃(wei)癌細胞

Raw264-7    小鼠單核細(xi)胞(bao)白(bai)血病細(xi)胞(bao)

SGC-7901    人胃癌細胞(bao)

SMMC-7721    人肝癌細(xi)胞

SNU-638    人胃癌(ai)細(xi)胞(bao)

SNU-739    人肝癌細胞

Tca8113    人舌鱗癌細胞    細胞系購(gou)買

U251    人腦(nao)膠質瘤細胞

U-373MG    人腦神經膠質瘤細胞

U-87 MG    人腦星形膠質母細胞(bao)瘤(liu)

BT-474    人乳(ru)腺導管癌細胞

PANC-1    人胰腺癌細胞

HEK293    人胚腎(shen)細(xi)胞

Hep3B    人肝癌(ai)細胞

HL-7702    人正常肝細胞

Raw-Blue    小鼠單核(he)細(xi)胞(bao)白血病細(xi)胞(bao)

WEHI-231    小鼠(shu)B淋巴細胞(bao)

OPM-2    人骨髓瘤細胞(bao)

NCI-H929    人骨髓瘤細胞

U266    人骨髓(sui)瘤細胞(bao)

EJ    人膀胱癌細胞

HOC    大鼠肝卵圓細胞

RWPE-1    人正(zheng)常(chang)前(qian)列腺(xian)上皮(pi)細胞

CAL 27    人舌(she)鱗癌(ai)細胞(bao)

Calu-3    人(ren)肺腺(xian)癌細胞

Eca-109    人食管(guan)癌(ai)細胞

DLD-1    人結直腸(chang)腺癌上(shang)皮細胞(bao)

5637.00     人膀胱癌細胞(bao)

SK-OV-3    人卵巢癌(ai)細(xi)胞

GBC-SD    人(ren)膽囊癌細(xi)胞

BEL7402 人肝癌(ai)細胞系

SW620    人結腸癌細胞

MRC-5    人胚肺(fei)成(cheng)纖維細胞     細胞系購買

PC9    人肺癌細(xi)胞(bao)

PC14    人(ren)肺(fei)癌細胞

BxPC    人原位胰腺腺癌細胞(bao)

PK（15）    豬腎上皮細胞(bao)

MDCK    狗腎上皮細胞

3D4/21    豬肺泡巨噬(shi)細胞

MHCC-97L    人肝癌細胞（低轉移）

MHCC-97H    人肝癌細胞(bao)系（高轉(zhuan)移）

MiaCaPa-2    人胰腺癌細胞

HN13    口腔(qiang)鱗狀細(xi)胞癌細(xi)胞

Bend3    小鼠腦微血管(guan)內皮細(xi)胞

QBC939    人膽管癌細胞

Ishikawa    人子宮(gong)內膜癌細胞(bao)

A498    人腎(shen)癌(ai)細胞

143B

WEHI-231    小(xiao)鼠B淋巴(ba)細胞

H322    人非小細(xi)胞肺癌細(xi)胞

Panc O2

S8    BEL7402 人肝癌細胞系(xi)

R5    細胞系(xi)購(gou)買

Pig MSC    豬骨髓(sui)間質干(gan)細胞(bao)

HCAEC    人(ren)冠狀(zhuang)動脈內(nei)皮細胞

marc-145    猴(hou)胚胎腎上皮細胞

3D4/21    豬肺泡巨噬細胞

PK（15）    豬腎上皮細胞

MADB-106    大鼠乳腺癌細胞

Sf9    昆蟲卵巢細胞

DADY

HUVEC[HUV-EC-C]    人(ren)臍靜脈內皮細(xi)胞

PC14    人肺癌細胞(bao)

PC9    人肺癌細胞

Eca-109    人食管癌(ai)細胞

CAL 27    人舌鱗癌細胞(bao)

HOC    肝卵圓細胞

EJ    人(ren)膀胱癌細胞

U266    人(ren)骨髓(sui)瘤(liu)細胞

293-mTLR5    人胚腎細胞

Raw-Blue    小(xiao)鼠單核(he)細胞(bao)白血病(bing)細胞(bao)

HL-7702    人正常肝細胞

Hep3B    人肝癌細(xi)胞(bao)

U-373MG    人腦神(shen)經膠質瘤細胞

Tca-8113    人舌鱗癌細(xi)胞

SNU-739    人肝癌細胞

SNU-638    人胃癌細胞

NUGC-3    人胃癌細胞

NIH-3T3    小鼠成纖維(wei)細(xi)胞

MKN-45    人胃(wei)癌細胞

LLC-PK1    豬腎近(jin)曲小管上皮細胞

Hs 746T    人胃癌細胞(bao)

Hepa1-6    小鼠肝癌細胞

HCT-8    人回盲腸癌細(xi)胞(bao)

BEL7402 人肝癌細(xi)胞系

HaCaT    人永(yong)生化(hua)表皮細胞

GES-1    人胃上皮(pi)細胞

B16-F0    小鼠黑色素瘤細胞

AZ-521    人胃癌細胞

786-O    人(ren)腎透明細胞腺癌細胞

208F    大鼠成纖維細胞

293TN    人(ren)胚腎細胞--用于慢病毒包裝

293FT    人胚腎細胞-用于慢病毒包裝

GP-293    人(ren)胚(pei)腎(shen)細胞

293-L.P.    人胚(pei)腎細胞

HTB-55 Calu-3 人類肺腺(xian)癌(ai) （胸水(shui)）














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