HeLa 229

ATCC® Number:  ;CCL-2.1™      Price:  $323.00

Designations:

HeLa 229  

Depositors:   JT Syverton

Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent (The line can be readily adapted to growth in suspension)

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: cervix

Disease: adenocarcinoma

Cellular Products: keratin

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Virus Susceptibility: Human adenovirus 7

Vesicular stomatitis virus

Human poliovirus 1

Human poliovirus 2

Human poliovirus 3

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes:  G6PD, A

Age:  31 years

Gender:  female

Ethnicity:  Black

HeLa Markers:  Y

Comments: Relatively resistant to polioviruses.

The cells are positive for keratin by immunoperoxidase staining.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C