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中文名稱人惡性非霍奇金淋巴瘤患者的自然殺傷細胞；NK-92MI英文名稱(cheng)NK-92MI規格

• T25

價格

• 2000

形(xing)態特性(xing)(xing)(xing)聚團生長特性(xing)(xing)(xing)懸浮(fu)特征(zheng)特性(xing)(xing)(xing)NK-92細(xi)(xi)(xi)(xi)(xi)(xi)胞是(shi)從(cong)一位患(huan)有急(ji)進性(xing)(xing)(xing)非(fei)霍(huo)奇(qi)金淋巴瘤(liu)的(de)(de)(de)(de)50歲白人男(nan)性(xing)(xing)(xing)外周血(xue)(xue)單核細(xi)(xi)(xi)(xi)(xi)(xi)胞衍生來的(de)(de)(de)(de)一株(zhu)(zhu)(zhu)IL-2依(yi)賴(lai)(lai)型NK細(xi)(xi)(xi)(xi)(xi)(xi)胞株(zhu)(zhu)(zhu)。NK-92MI細(xi)(xi)(xi)(xi)(xi)(xi)胞是(shi)轉(zhuan)染(ran)得(de)到的(de)(de)(de)(de)源自(zi)NK-92細(xi)(xi)(xi)(xi)(xi)(xi)胞的(de)(de)(de)(de)IL-2非(fei)依(yi)賴(lai)(lai)的(de)(de)(de)(de)NK細(xi)(xi)(xi)(xi)(xi)(xi)胞株(zhu)(zhu)(zhu)。親(qin)本細(xi)(xi)(xi)(xi)(xi)(xi)胞NK-92通過微粒體基(ji)因(yin)轉(zhuan)化(hua)法用(yong)逆(ni)轉(zhuan)錄(lu)病毒MFG-hIL-2載體攜帶的(de)(de)(de)(de)人IL-2cDNA進行(xing)轉(zhuan)化(hua)。可(ke)(ke)能由于載體整合到基(ji)因(yin)組DNA中，轉(zhuan)化(hua)是(shi)穩定的(de)(de)(de)(de)。這株(zhu)(zhu)(zhu)細(xi)(xi)(xi)(xi)(xi)(xi)胞對(dui)很多(duo)惡性(xing)(xing)(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞有細(xi)(xi)(xi)(xi)(xi)(xi)胞毒性(xing)(xing)(xing)；鉻釋放(fang)試(shi)驗顯示它能殺死(si)K562細(xi)(xi)(xi)(xi)(xi)(xi)胞和(he)Daudi細(xi)(xi)(xi)(xi)(xi)(xi)胞。NK-92細(xi)(xi)(xi)(xi)(xi)(xi)胞有以(yi)下特征(zheng)：CD2、CD7、CD11a、CD28、CD45、CD54表(biao)面標記陽性(xing)(xing)(xing)；CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和(he)HLA-DR表(biao)面標記陰性(xing)(xing)(xing)。其親(qin)本IL-2依(yi)賴(lai)(lai)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞株(zhu)(zhu)(zhu)NK-92細(xi)(xi)(xi)(xi)(xi)(xi)胞及另一株(zhu)(zhu)(zhu)同(tong)樣來源于NK-92細(xi)(xi)(xi)(xi)(xi)(xi)胞株(zhu)(zhu)(zhu)的(de)(de)(de)(de)IL-2非(fei)依(yi)賴(lai)(lai)的(de)(de)(de)(de)細(xi)(xi)(xi)(xi)(xi)(xi)胞株(zhu)(zhu)(zhu)NK-92CI都(dou)可(ke)(ke)從(cong)ATCC得(de)到。NK-92MI細(xi)(xi)(xi)(xi)(xi)(xi)胞和(he)NK-92CI細(xi)(xi)(xi)(xi)(xi)(xi)胞這兩個(ge)變(bian)種(zhong)都(dou)包含(han)、表(biao)達(da)并合成(cheng)hIL-2cDNA。NK-92MI細(xi)(xi)(xi)(xi)(xi)(xi)胞合成(cheng)的(de)(de)(de)(de)IL-2水平比NK-92CI高，而親(qin)本細(xi)(xi)(xi)(xi)(xi)(xi)胞不合成(cheng)表(biao)達(da)。1998年9月提交到ATCC的(de)(de)(de)(de)培(pei)養(yang)(yang)物(wu)污(wu)染(ran)了支原(yuan)體，其后(hou)代通過BM細(xi)(xi)(xi)(xi)(xi)(xi)胞周期(qi)蛋(dan)白處理21天消除(chu)支原(yuan)體。處理后(hou)6周，用(yong)Hoechst染(ran)色、PCR和(he)標準(zhun)培(pei)養(yang)(yang)測(ce)(ce)試(shi)進行(xing)支原(yuan)體檢測(ce)(ce)，結(jie)果都(dou)呈陰性(xing)(xing)(xing)。培(pei)養(yang)(yang)條件(jian)(jian)MEMα＋0.2mM Inositol＋0.1mM β-mercaptoethanol＋0.02mM Folic Acid＋12.5% HS＋12.5% FBS傳(chuan)代方法1:2凍存(cun)條件(jian)(jian)無血(xue)(xue)清凍存(cun)液