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HMy2.CIR 人B淋巴母細胞
IMDM培養基(GIBCO)+10%胎(tai)牛血清 細胞貨期8-10個(ge)工作(zuo)日
上海復祥生物提供(gong) ATCC 細(xi)(xi)(xi)(xi)胞|細(xi)(xi)(xi)(xi)胞系|細(xi)(xi)(xi)(xi)胞株|腫瘤細(xi)(xi)(xi)(xi)胞|細(xi)(xi)(xi)(xi)胞|貼壁(bi)細(xi)(xi)(xi)(xi)胞|懸浮細(xi)(xi)(xi)(xi)胞|,細(xi)(xi)(xi)(xi)胞庫管理規范,提供(gong)的細(xi)(xi)(xi)(xi)胞株背景(jing)清楚(chu),提供(gong)參考文獻和(he)培養條(tiao)件,上有(you)細(xi)(xi)(xi)(xi)胞照(zhao)片,歡迎各位老師! 手(shou)機(ji)
培養基 The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
傳代方法 Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 viable cells/mL.
Interval: Maintain between 2 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
生長條件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
生長特性 suspension
存儲條件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v
) DMSO
Storage temperature: liquid nitrogen vapor phase
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