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VERO 非洲綠猴腎細胞

簡要(yao)描述(shu):VERO 非洲綠猴腎細胞
ATCC 細(xi)胞(bao)|細(xi)胞(bao)系|細(xi)胞(bao)株(zhu)(zhu)|腫瘤細(xi)胞(bao)|細(xi)胞(bao)|貼壁(bi)細(xi)胞(bao)|懸浮細(xi)胞(bao)|,細(xi)胞(bao)庫管理規(gui)范(fan),提供(gong)的細(xi)胞(bao)株(zhu)(zhu)背景清楚,提供(gong)參考文獻和*培養條件

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VERO C1008 (E6)非洲綠猴腎細胞

ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件

VERO 非洲綠猴腎細胞的詳細介紹


ATCC® Number:CRL-1586™  Price:
Designations:VERO C1008 [Vero 76, clone E6, Vero E6]

Depositors:EM Earley

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Cercopithecus aethiops

Morphology:epithelial

Source:Organ: kidney
                       Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Susceptibility:Junin virus

Machupo virus

Lassa virus

Marburg virus

Zaire Ebola virus



Comments:This is a clone of VERO 76 (ATCC CRL-1587).

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                       Temperature: 37.0°C


Subculturing:Protocol:                        
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
                       Medium Renewal: 2 to 3 times per week


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
                       Storage temperature: liquid nitrogen vapor phase


Doubling Time:22 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988

32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020


















 

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