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美國MEFs Oct4-GFP報告基因小鼠胚胎成纖維細胞
美國PrimCells干細胞：PrimCells提(ti)供多(duo)種報(bao)告(gao)基因敲進的(de)小(xiao)鼠(shu)胚胎干(gan)細胞(bao)，該類細胞(bao)皆在(zai)原始基因座(zuo)上敲入(ru)熒光報(bao)告(gao)基因，從而使熒光蛋白的(de)表達能真實反映原基因座(zuo)附近(jin)的(de)表達調(diao)控狀況。該類細胞(bao)作為研究(jiu)胚胎干(gan)細胞(bao)自更(geng)新和定向分(fen)化的(de)有力工具，受到多(duo)家著名研究(jiu)所研究(jiu)人員(yuan)的(de)青睞。
| Species: | Mus Musculus (129/B6 Mix) | Catalog Number: | PCEMM03 |
| Cell Number: | ~1x106 | Storage/Shipping: | Dry Ice |
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美國MEFs Oct4-GFP報告基因小鼠胚胎成纖維細胞
Introduction:
Primary mouse embryonic fibroblasts (MEFs) are typically derived from E13.5 mouse embryos. They are relatively easy to culture and maintain and have robust proliferation rate. MEFs can be powerful tools to study gene functions especially when comparison experiments between wild-type and knockout cells from the same liter are required. Meanwhile, MEFs have also been used as feeder cells to support growth of embryonic stem cells from a variety of species1. More recently, MEFs from reporter transgenic mice were used for assessing gene function during reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) 2.
PrimCells is proud to isolate and provide the primary cultures of embryonic fibroblasts from transgenic mice carrying IRES-GFP gene in their endogenous Pou5f1 (Oct4) locus. When these cells are fully reprogrammed, iPSCs will become GFP+, which could be a powerful tool for chemical screening or gene functional studies in order to develop more efficient reprogramming technology.
Figure 1 | Successfully reprogrammed iPSCs become GFP positive
美國PrimCells品牌介紹
PrimCells是位于美國加州圣迭戈的一家專業致力于細胞產品研發和解決方案提供的業內技術。
公司匯集了資深的生物醫藥技術專家，獨立開發了多項原代細胞和干細胞相關的專有技術。 公司以“細胞研究的優選擇"為宗旨，自主研發超過70種創新產品，多個特種細胞市場的空白。例如其誘導性表達細胞庫和高效3D皮膚培養系統，皆為該類產品目前市場上少數的規模化供應商。此外，公司以高質量的數據與快速穩定的項目完成時間，長期為多家制藥公司提供解決方案，獲得用戶廣泛贊譽。
PrimCells產品線：
1. 報告基因干細胞 2. 原代細胞（含報告基因原代細胞） 3. 誘導性穩定細胞株
4. 人源重組蛋白 5. 創新型培養試劑
PrimCells 解決方案
1.誘導性穩定細胞株開發 2. 原代細胞開發 3. 藥物體外毒理和效能測試 4. 3D-皮膚培養
PrimCells合作伙伴及客戶:
A．生物制藥公司：輝瑞(Pfizer), 諾華(Novartis), 禮來(Eli2 Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和醫藥研發部門
B．科研機構：冷泉港實驗室，哈佛醫學院, 麻省理工（MIT）,， UCSF, NYU, Salk, UIUC, USC等
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