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簡要(yao)描述:美國小鼠Nurr1可誘導表達穩定細胞株美國PrimCells誘(you)導(dao)(dao)性(xing)穩定(ding)細胞(bao)(bao)(bao)株: PrimCells建立的(de)(de)*的(de)(de)誘(you)導(dao)(dao)性(xing)細胞(bao)(bao)(bao)高(gao)效開發(fa)系統,能在極(ji)短時間內篩(shai)選出(chu)低(di)泄漏,高(gao)誘(you)導(dao)(dao)性(xing)的(de)(de)穩定(ding)細胞(bao)(bao)(bao)株,成(cheng)功開發(fa)出(chu)一系列核受體誘(you)導(dao)(dao)表達(da)的(de)(de)穩定(ding)細胞(bao)(bao)(bao)株產品,為多家(jia)制藥公司研發(fa)部(bu)門和科研院所提供(gong)產品與服務,廣受用戶好評(ping)。針對(dui)客戶的(de)(de)特(te)定(ding)需求,PrimCells亦提供(gong)量身定(ding)制服務
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美國小鼠Nurr1可誘導表達穩定細胞株的說明:
PrimCells
美國小鼠Nurr1可誘導表達穩定細胞株: PrimCells建立的*的誘導性細胞高效開發系統,能在極短時間內篩選出低泄漏,高誘導性的穩定細胞株,成功開發出一系列核受體誘導表達的穩定細胞株產品,為多家制藥公司研發部門和科研院所提供產品與服務,廣受用戶好評。針對客戶的特定需求,PrimCells亦提供量身定制服務,通過密切的客戶交流和高效的篩選體系,嚴格保證客戶在3-4周內即獲得高質量的誘導性穩定細胞株,具有市場其他供應商*的時效性。
Introduction:
Nuclear hormone receptors (NHRs) are a superfamily of transcription factors that function as powerful metabolic regulators to control a variety of systemic processes in physiology. They also play key roles in the pathophysiology of many major disease states, such as diabetes, obesity, inflammation, atherosclerosis and heart failure. To date, this superfamily has provided a rich source of drug design targets and it is continuing to be one of the hottest areas for pharmaceutical researches.
PrimCells now provides a comprehensive set of NHR expressing cell lines to the research community. These high-quality, flag-and/or HA-tagged NHR-expressing cell lines aim to facilitate further biochemical and molecular studies of their functions and hence encourage new strategies for drug design.
The mouse Nurr1 stable cell line was created by stable co-transfection of HEK293 cells with two plasmids expressing the bacterial Tet repressor (TetR) and the mouse Nurr1 protein with FLAG tag, respectively. Since the mouse Nurr1 gene is driven by a promoter containing two tetracycline operator (TeTO2) sites, its expression can be induced by doxycycline.
美國PrimCells品牌介(jie)紹
PrimCells是位(wei)于美(mei)國加(jia)州圣迭戈的一(yi)家專(zhuan)業致力(li)于細胞產品研發(fa)和解(jie)決(jue)方案提供的業內技術。
公司(si)(si)匯集了資深的(de)生物醫藥(yao)技(ji)術專(zhuan)家,獨立開發了多項原(yuan)代(dai)細(xi)胞(bao)和干細(xi)胞(bao)相關的(de)專(zhuan)有技(ji)術。 公司(si)(si)以“細(xi)胞(bao)研(yan)究的(de)優選擇"為宗(zong)旨,自主研(yan)發超(chao)過(guo)70種創新(xin)產品(pin),多個特種細(xi)胞(bao)市(shi)場的(de)空白。例如其誘導性表達細(xi)胞(bao)庫和高效3D皮膚培養系(xi)統,皆(jie)為該類產品(pin)目前市(shi)場上少數(shu)的(de)規模化供應商。此外,公司(si)(si)以高質量的(de)數(shu)據與快速穩定的(de)項目完(wan)成(cheng)時間,長期為多家制(zhi)藥(yao)公司(si)(si)提供解(jie)決(jue)方案(an),獲得用戶廣泛贊譽(yu)。
PrimCells產品(pin)線:
報告基(ji)(ji)因干細(xi)胞 2. 原代細(xi)胞(含(han)報告基(ji)(ji)因原代細(xi)胞) 3. 誘導性穩(wen)定細(xi)胞株(zhu)
4. 人源重組蛋白 5. 創(chuang)新型培養試劑
PrimCells 解決方案
1.誘導性穩定細(xi)胞株開發 2. 原代細(xi)胞開發 3. 藥物體外毒理和效能測試(shi) 4. 3D-皮膚(fu)培(pei)養
PrimCells合(he)作伙伴(ban)及客戶:
A.生物制藥公(gong)司:輝瑞(Pfizer), 諾華(Novartis), 禮來(Eli2 Lilly),. Amgen, EMD Millipore, Regeneron等公(gong)司的毒理(li)和醫藥研發部門
B.科(ke)研機構:冷泉港實(shi)驗室,哈(ha)佛醫學院, 麻(ma)省理(li)工(MIT),, UCSF, NYU, Salk, UIUC, USC等
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