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Pt K1 (NBL-3) 袋鼠腎細胞

簡要(yao)描述:CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
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  • 產品(pin)型號(hao):CCL-35
  • 廠商性質(zhi):生產廠家
  • 更新(xin)時間:2023-11-21
  • 訪  問  量:1688

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詳細介紹

CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件

CCL-35 Pt K1 (NBL-3) 袋鼠腎細胞的詳細介紹

CCL-35

ATCC® Number:CCL-35™  Price:$438.00
Designations:Pt K1 (NBL-3) [PTK1]

Depositors:KH Walen

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Potorous tridactylis (potoroo)

Morphology:epithelial


Source:Organ: kidney
Disease: normal


Cellular Products:keratin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Resistance:poliovirus 2

Age:adult

Gender:female

Comments:The cells are positive for keratin by immunoperoxidase staining.

Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%

Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week

Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.



References:26188: . . Nature 194: 406, 1962.

26189: . . Cytogenetics 3: 19, 1964.















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